Qualidade seminal e histomorfometria dos órgãos reprodutivos de ovinos tratados com fluoreto de sódio Seminal quality and reproductive histomorphometry of ram lambs treated with sodium fluoride
| Autor: | Andreane Filappi, Danívia Prestes, Ricardo Rocha, Deila Rosely Schosller, Carlos Bondan, José Francisco Bragança, Marcelo Cecim |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 38, Iss 9, Pp 2561-2566 (2008) |
| Druh dokumentu: | article |
| ISSN: | 0103-8478 1678-4596 |
| DOI: | 10.1590/S0103-84782008000900024 |
| Popis: | O presente estudo objetivou avaliar as características espermáticas, os indicadores do metabolismo oxidativo do sêmen, o diâmetro dos túbulos seminíferos e a altura do epitélio testicular, do epididimário e do canal deferente de ovinos tratados com fluoreto de sódio. Foram utilizados 12 ovinos, com idade aproximada de cinco meses. Os animais foram divididos em grupo controle, o qual recebeu diariamente sal iodado (5g de NaCl animal-1 + 0,2mg I kg-1 matéria seca), e tratado, que recebeu sal iodado adicionado de fluoreto de sódio (4,7mg F kg-1 de peso corporal). Aos 150 dias de tratamento, foram realizadas coletas de sêmen e, em seguida, os animais foram eutanasiados. Na comparação entre o grupo controle e o tratado, não foram observadas diferenças no percentual de motilidade, vivos : mortos e morfologia espermática, na concentração da glutationa reduzida seminal, no teor de zinco seminal, no peso testicular, na morfometria e na histologia do testículo, na cauda do epidídimo e no canal deferente. A concentração de substâncias reativas ao ácido tiobarbitúrico (TBARs) e o teor de cobre no sêmen foram menores (PThe objective of the present study was to evaluate different reproductive parameters of ram lambs treated with sodium fluoride. Spermogram, indicators of seminal oxidative profile, seminiferous tubules diameter and cell height of the testicular epithelium, epididimus and vas deferens were analyzed. Twelve 5-month-old ram lambs were used. They were allocated into one of two experimental groups: The control, which received daily iodized salt (5g NaCl animal-1 + 0.2mg I kg-1 DM); and the treated, which received (5g NaCl animal-1 + 0.2mg I kg-1 DM + 4.7mg F kg-1 BW). After 150 days of treatment, animals went through a complete andrological exam, and were euthanized on the next day. No differences were observed in sperm motility, dead: alive ratio and morphology between the control and treated group. Also, seminal concentration of reduced glutathione and zinc, as well as, testicular weight, histology and morphometry of testicles, caudal portion of epididimus and vas deferens did not differ between groups. The seminal concentration of thiobarbituric acid reactive substances (TBARs) and seminal copper levels were lower (P |
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