Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

Autor: María F. Pomponio, Cintia Acuña, Vivien Pentreath, Diego L. Lauenstein, Susana M. Poltri, Susana Torales
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Forest Systems, Vol 24, Iss 2, Pp eRC04-eRC04 (2015)
Druh dokumentu: article
ISSN: 2171-5068
2171-9845
DOI: 10.5424/fs/2015242-07188
Popis: Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P.alba, P. denudans, P. hassleriP. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.
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