| Autor: |
Stephan G Anagnostaras, Suzanne C Wood, Tristan Shuman, Denise J Cai, Arthur D LeDuc, Karl R Zurn, J. Brooks Zurn, Jennifer R Sage, Gerald M Herrera |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Frontiers in Behavioral Neuroscience, Vol 4 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
1662-5153 |
| DOI: |
10.3389/fnbeh.2010.00158 |
| Popis: |
The Pavlovian conditioned freezing paradigm has become a prominent mouse and rat model of learning and memory, as well as of pathological fear. Due to its efficiency, reproducibility, and well-defined neurobiology, the paradigm has become widely adopted in large-scale genetic and pharmacological screens. However, one major shortcoming of the use of freezing behavior has been that it has required the use of tedious hand scoring, or a variety of proprietary automated methods that are often poorly validated or difficult to obtain and implement. Here we report an extensive validation of the Video Freeze system in mice, a turn-key all-inclusive system for fear conditioning in small animals. Using digital video and near-infrared lighting, the system achieved outstanding performance in scoring both freezing and movement. Given the large-scale adoption of the conditioned freezing paradigm, we encourage similar validation of other automated systems for scoring freezing, or other behaviors. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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