Autor: |
Martino Bardelli, Francisco Zarate-Perez, Leticia Agundez, Nelly Jolinon, R. Linden, Carlos Escalante, Els Henckaerts |
Jazyk: |
angličtina |
Rok vydání: |
2017 |
Předmět: |
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Zdroj: |
Bio-Protocol, Vol 7, Iss 9 (2017) |
Druh dokumentu: |
article |
ISSN: |
2331-8325 |
DOI: |
10.21769/BioProtoc.2271 |
Popis: |
Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates can be detected. This detailed protocol describes how to analyze the AAV DNA intermediates formed during AAV replication using a modified Hirt extract (Hirt, 1967) procedure and Southern blotting (Southern, 1975). |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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