| Autor: |
Peizhen Tao, Wei Jiang, Liyan Yu, Minghua Chen, Shuoke Bai, Yexiang Wu, Xiuyong Fan, Yuqin Zhang, Yongzhen Li, Tian Zhang, Biao Dong, Maoluo Gan, Xiao Liu, Shuyi Si |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Molecules, Vol 18, Iss 1, Pp 236-243 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1420-3049 |
| DOI: |
10.3390/molecules18010236 |
| Popis: |
We have isolated an extraordinary pentapeptide, called 4862F, from the culture broth of Streptomyces albosporus I03A-04862 by Diaion HP-20 macroporous adsorbent resin column, ODS-A and Sephadex LH-20 chromatography, followed by preparative HPLC. This peptide shows inhibitory activity against HIV-1 protease. The structure was elucidated by spectroscopic approaches, including ESI-MS and various NMR methods. Absolute configuration of the amino acid residues in 4862F was defined using Marfey’s method, and the structure was identified as N,N,N-(trimethylated)-Tyr-L-Leu-L-Val-L-Leu-(dehydrated)-His. The peptide 4862F displays inhibitory activity against HIV-1 protease, with IC50 values of 15.26 nM, using a fluorescence-based assay. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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