Effects of ultrasound on the enzymatic degradation of pectin

Autor: Lena Rebecca Larsen, Judith van der Weem, Rita Caspers-Weiffenbach, Andreas Schieber, Fabian Weber
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Ultrasonics Sonochemistry, Vol 72, Iss , Pp 105465- (2021)
Druh dokumentu: article
ISSN: 1350-4177
DOI: 10.1016/j.ultsonch.2021.105465
Popis: Ultrasound-assisted enzymatic maceration (UAEM) has gained considerable interest in the fruit juice industry, owing to its potential to increase juice yield and content of polyphenols while simultaneously saving time and energy. In this study, the effects of UAEM (ultrasonic probe, 20 kHz, 21 W*cm−2 and 33 W*cm−2) on pectin degradation in a continuous circulation system were investigated over 60 and 90 min. Main pectinolytic enzymes activities of (polygalacturonase, pectin lyase and pectin methylesterase) of a commercial enzyme preparation were examined for individual synergistic effects with US. Pectin hydrolysis by UAEM differed significantly compared to treatment with ultrasound or enzymes alone regarding the profile of degradation products compared to treatment with ultrasound or enzymes alone. Ultrasound fragmented pectin to less branched oligomers of medium molecular weight (Mp approx. 150 kDa), which were further degraded by pectinolytic activities. The low molecular weight fraction (
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