Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus

Autor: Feng-shan Gao, Lei Feng, Ping Jiang, Zi-bin Li, Hua Gao, Xiao-xin Zhai, Zong-hui Zhang, Xiao Hu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: BMC Veterinary Research, Vol 14, Iss 1, Pp 1-6 (2018)
Druh dokumentu: article
ISSN: 1746-6148
DOI: 10.1186/s12917-018-1742-3
Popis: Abstract Background Currently, the structural characteristics of the swine major histocompatibility complex (MHC) class I molecule, also named swine leukocyte antigen class I (SLA-I) molecule need to be further clarified. Results A complex of SLA-I constituted by an SLA-2*HB01 molecule with swine β2-microglobulin and a cytotoxic T lymphocyte (CTL) epitope FMDV-AS64 (ALLRSATYY) derived from VP1 protein (residues 64–72) of Asia 1 serotype of foot-and-mouth disease virus (FMDV) was expressed, refolded, purified and crystallized. By preliminary X-ray diffraction analysis, it was shown that the diffraction resolution of the crystal was 2.4 Å and the space group belonged to P212121 with unit cell parameters a = 48.37, b = 97.75, c = 166.163 Å. Conclusion This research will be in favor of illuminating the structural characteristics of an SLA-2 molecule associated with a CTL epitope derived from Asia1 serotype of FMDV.
Databáze: Directory of Open Access Journals
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