| Autor: |
Xin Xu, Qin Luo, Jixiang Wang, Yahui Song, Hong Ye, Xin Zhang, Yi He, Minxuan Sun, Ruobing Zhang, Guohua Shi |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Opto-Electronic Advances, Vol 7, Iss 6, Pp 1-16 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2096-4579 |
| DOI: |
10.29026/oea.2024.230212 |
| Popis: |
Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view, typically around 1 mm. This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample, such as different brain areas. In addition, conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo. Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm, a numerical aperture of 0.5, and a working wavelength range from 400 to 1000 nm. We achieved a resolution of 0.74 μm in fluorescent beads imaging. The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system, a confocal laser scanning system, and a two-photon imaging system. This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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