| Autor: |
Hui Wen, Yun Zhang, Xi-Luan Ji, Xiao-Lei Liu, Shun Yang, Zhao-Xia Luo, Liang Xie, Wen-Wen Zou, Jie-Ming Li, Shu Jiang |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Guoji Yanke Zazhi, Vol 17, Iss 7, Pp 1226-1231 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1672-5123 |
| DOI: |
10.3980/j.issn.1672-5123.2017.7.07 |
| Popis: |
AIM: To construct the recombined pLenti-CMV-PEDF-EYFP vector to be infected into human umbilical cord-derived mesenchymal stem cells(hUCMSCs), and then to identify the PEDF expression in hUCMSCs and the cell viability of hUCMSCs after infection with the recombined lentivirus. METHODS: The recombinant pLenti-CMV-PEDF-EYFP vector was constructed with pLenti-CMV-hChR2-EYFP and PEDF gene, and then infected into hUCMSCs to obtain the PEDF-hUCMSCs. The expression of PEDF in hUCMSCs was identified by confocal microscopy and ELISA. The Cell Counting Kit(CCK8)was used to assess the cell viability of PEDF-hUCMSCs. The influences of pLenti-CMV-PEDF-EYFP infection on the passage and phenotypes of hUCMSCs were assessed by microscope and flow cytometry. RESULTS: The recombinant pLenti-CMV-PEDF-EYFP was successfully constructed and efficiently infected into hUCMSCs. The expression of PEDF was positively detected in the PEDF-hUCMSCs. No significant difference was observed on cell viability between PEDF-modified hUCMSCs and hUCMSCs(P>0.05). The infection of pLenti-CMV-PEDF-EYFP had no obvious influences on the proliferation, morphology and phenotypes of hUCMSCs. CONCLUSION: PEDF was expressed effectively in the hUCMSCs modified with the recombinant lentivirus. The preliminary results from this study provide more evidences for further study of using PEDF-hUCMSCs to treat retinitis pigmentosa. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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