Antioxidant Potential of Vitex Doniana Ethanol Leaf Extract In Triton X-100induced Hyperlipidemic Wistar Albino Rats
| Autor: | C. O. Okpala, I. O. Igwilo, F. Eze |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | The Bioscientist, Vol 9, Iss 1 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2630-7103 2630-7111 |
| Popis: | This study was undertaken to investigate the antioxidant potential of ethanol leaf extract of Vitex doniana against Triton X-100 induced hyperlipidaemia in rats. The percentage yield of the leaf was 9.21% w/w. The phytochemical constituents of the extract were identified using the standard method of AOAC. In vitro antioxidant activity of the extract (0.1–0.5 mg/mL) was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, and nitric oxide radical. In vivo antioxidant potential of the extract was evaluated in triton X-100 induced hyperlipidemic and normal rats. The LD50 of the leaves extract was greater than 5000mg/kg body weight. Quantitative analysis of the plant secondary metabolites showed the presence of tannin (32.40±0.02 mg/g), flavonoid (16.87±0.27 mg/g), alkaloid (0.50±0.07 mg/g), cyanogenic glycosides (0.06±0.00 µg/g) and saponin (0.83±0.10mg/g). The result of proximate composition showed the presence of carbohydrate (41.85±0.73 %), protein (19.26±0.12%), fats (12.75±0.41%), moisture (12.04±0.17 %), crude fibre (11.82±0.19 %) and ash (11.90±1.00 %). The extract scavenged DPPH and nitric oxide radical concentration dependently. A total of thirty (30) adult male albino rats were divided into six (6) groups (A, B, C, D, E, and F), with five (5) animals in each group. Group A and B received distilled water only; Group C received 10 mg/kg b. w of atorvastatin while groups D, E, and F received an oral administration of 200, 400 and 600 mg/kg b.w. of Vitex doniana leaf extract, respectively. After 21 days, their blood serums were analysed. A significant (p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|