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Entomopathogenic fungi (EPF) consist of a wide range of fungi that can be used as pest control agents, endophytes, and plant growth promoters. In this study of EPF in suburban soils from Achaia, Greece, we used adult beetles as baits for trapping fungal isolates. According to the macroscopic and microscopic traits of the collected isolates, three species corresponded to Beauveria varroae Vuill. (Hypocreales: Cordycipitaceae); Purpureocillium lavendulum Perdomo, Gené, Cano & Guarro (Hypocreales: Ophiocordycipitaceae); and Cordyceps blackwelliae Mongkolsamrit, Noisripoom, Thanakitpipattana, Spatafora & Luangsaard (Hypocreales: Claviceptaceae). Their taxonomic identity was established by ITS-rDNA sequence amplification and sequencing, molecular database comparisons, and phylogenetic analysis. The application of these new EPF species clearly demonstrated remarkable insecticidal action on Thaumetopoea pityocampa (Lepidoptera, Notodontidae) larvae, which increased with the application dose. Our findings are important based on the enhancement of the application of new EPF species as biocontrol agents within the framework of eco-friendly pest management. |
