Autor: |
Pasaikou Sonko, Solomon Chih-Cheng Chen, Chia-Mei Chou, Ying-Chieh Huang, Shao-Lun Hsu, Daniel Barčák, Mikuláš Oros, Chia-Kwung Fan |
Jazyk: |
angličtina |
Rok vydání: |
2020 |
Předmět: |
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Zdroj: |
Journal of Microbiology, Immunology and Infection, Vol 53, Iss 6, Pp 1021-1029 (2020) |
Druh dokumentu: |
article |
ISSN: |
1684-1182 |
DOI: |
10.1016/j.jmii.2019.04.012 |
Popis: |
Background: Anisakid larvae are the food-borne pathogen highly prevalent among numerous marine fishes. Accidental consumption of infected raw or poorly cooked fish fillets may cause anisakiasis. Methods: This study used the multidisciplinary approach to investigate the occurrence of Anisakis nematodes in commonly consumed fish species, Scomber australasicus and Trichiurus lepturus purchased in Taipei Xinyi traditional fish market. Results: All the Anisakis larvae collected herein were identified morphologically as Anisakis type I or Anisakis type II. The prevalence and the mean intensity of Anisakis larvae collected from S. australasicus was 80.77%, 26.8 (10–32) and 100%, 49.0 (27–70) for T. lepturus. Using molecular analysis, 83.33% (180/216) were identified as Anisakis pegreffii, 6.05% (13/216) as Ascaris typica, 1.85% (4/216) as Ascaris physeteris and 8.80% (19/216) as hybrid genotype (A. pegreffii + Anisakis simplex) in S. australasicus. In T. lepturus, 86.31% (290/336) were identified as A. pegreffii, 2.38% (8/336) as A. typica, and 11.31% (38/336) as hybrid genotype (A. pegreffii + A. simplex [s.s]). The molecular phylogenetic analysis shows two cluster clades, one group includes A. pegreffii complex and the other include Ascaris paggiae, Ascaris brevispiculata, and A. physeteris. Conclusion: Thus, A. pegreffii is the most abundant species and may be the potential causes of human infection. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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