Pooled DNA sequencing in hairy vetch (Vicia villosa Roth) reveals QTL for seed dormancy but not pod dehiscence

Autor: Neal Tilhou, Lisa Kissing Kucek, Brandon Carr, Joel Douglas, John Englert, Shahjahan Ali, John Raasch, Suresh Bhamidimarri, Steven Mirsky, Maria J. Monteros, Ryan Hayes, Heathcliffe Riday
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Frontiers in Plant Science, Vol 15 (2024)
Druh dokumentu: article
ISSN: 1664-462X
DOI: 10.3389/fpls.2024.1384596
Popis: IntroductionHairy vetch (Vicia villosa Roth) is a promising legume cover crop, but its use is limited by high rates of pod dehiscence and seed dormancy.MethodsWe used phenotypically contrasting pooled DNA samples (n=24 with 29-74 individuals per sample) from an ongoing cover crop breeding program across four environments (site-year combinations: Maryland 2020, Maryland 2022, Wisconsin 2021, Wisconsin 2022) to find genetic associations and genomic prediction accuracies for pod dehiscence and seed dormancy. We also combined pooled DNA sample genetic association results with the results of a prior genome-wide association study.Results and discussionGenomic prediction resulted in positive predictive abilities for both traits between environments and with an independent dataset (0.34-0.50), but reduced predictive ability for DNA pools with divergent seed dormancy in the Maryland environments (0.07-0.15). The pooled DNA samples found six significant (false discovery rate q-value
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