Production and Sensing of Butyrate in a Probiotic E. coli Strain

Autor:	Yanfen Bai, Thomas J. Mansell
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	metabolic engineering E. coli Nissle 1917 short-chain fatty acids biosensor Biology (General) QH301-705.5 Chemistry QD1-999
Zdroj:	International Journal of Molecular Sciences, Vol 21, Iss 10, p 3615 (2020)
Druh dokumentu:	article
ISSN:	21103615 1422-0067 1661-6596
DOI:	10.3390/ijms21103615
Popis:	The short-chain fatty acid butyrate plays critical roles in human gut health, affecting immunomodulation, cell differentiation, and apoptosis, while also serving as the preferred carbon source for colon cells. In this work, we have engineered a model probiotic organism, E. coli Nissle 1917 (EcN, serotype O6:K5:H1), to produce butyrate from genomic loci up to approximately 1 g/L (11 mM). Then, for real-time monitoring of butyrate production in cultures, we developed a high-throughput biosensor that responds to intracellular butyrate concentrations, with green fluorescent protein as the reporter. This work provides a foundation for studies of butyrate for therapeutic applications.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/bc5b23bd185a4504a08ed4e23810714c Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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