Does the time between collecting and processing umbilical cord blood samples affect the quality of the sample?

Autor: Ricardo Barini, Ubirajara Costa Ferraz, Gregório Lorenzo Acácio, Isabela Nelly Machado
Jazyk: English<br />Portuguese
Rok vydání: 2011
Předmět:
Zdroj: Einstein (São Paulo), Vol 9, Iss 2, Pp 207-211 (2011)
Druh dokumentu: article
ISSN: 1679-4508
Popis: Objective: To assess the association between the time fromumbilical cord blood collection until processing and the quality of the sample. Methods: Umbilical cord blood samples collected duringthe third stage of labor were placed in temperature-controlled boxesfor the transport of biological material and sent to an umbilical cordblood bank, where the number of nucleated cells, viable cells andCD34+ cells were counted, and samples were additionally testedfor contamination at the following time intervals: up to 24 hours,up to 48 hours and up to 72 hours following sampling. Data wereanalyzed using the multivariate analysis of variance (MANOVA) andcompared using McNemar’s χ2 test. Significance was defined at p< 0.05. Results: Means and medians of the number of nucleatedcells, viable cells and CD34+ cells decreased significantly (p
Databáze: Directory of Open Access Journals