| Autor: |
J.T. Carreira, J.T. Trevizan, B.H. Kipper, S.H.V. Perri, I.R. Carvalho, L.H. Rodrigues, C. Silva, M.B. Koivisto |
| Jazyk: |
English<br />Portuguese |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 67, Iss 2, Pp 417-423 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1678-4162 |
| DOI: |
10.1590/1678-7046 |
| Popis: |
The routine semen evaluation assessing sperm concentration, motility and morphology, does not identify subtle defects in sperm chromatin architecture. Bulls appear to have stable chromatin, with low levels of DNA fragmentation. However, the nature of fragmentation and its impact on fertility remain unclear and there are no detailed reports characterizing the DNA organization and damage in this species. The intensive genetic selection, the use of artificial insemination and in vitro embryo production associated to the cryopreservation process can contribute to the chromatin damage and highlights the importance of sperm DNA integrity for the success of these technologies. Frozen-thawed semen samples from three ejaculates from a Nellore bull showed high levels of morphological sperm abnormalities (55.8±5.1%), and were selected for complementary tests. Damage of acrosomal (76.9±8.9%) and plasma membranes (75.7±9.3%) as well as sperm DNA strand breaks (13.8±9.5%) and protamination deficiency (3.7±0.6%) were significantly higher compared to the values measured in the semen of five Nellore bulls with normospermia (24.3±3.3%; 24.5±6.1%; 0.6±0.5%; 0.4±0.6% for acrosome, plasma membrane, DNA breaks and protamine deficiency, respectively) (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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