| Autor: |
ZHANG Meijin, WANG Lan, ZHONG Yu, GUAN Yongyi, JIN Meng, LIN Haidan |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Zhongguo shipin weisheng zazhi, Vol 35, Iss 9, Pp 1290-1296 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1004-8456 |
| DOI: |
10.13590/j.cjfh.2023.09.006 |
| Popis: |
ObjectiveA method involving derivatization combined with liquid chromatography-tandem mass spectrometry was developed for the determination of vitamin D in infant formula food.MethodsThe samples were treated with enzymolysis， saponification， liquid-liquid extraction， and 4-phenyl-1，2，4-triazoline-3，5-dion derivatization. Chromatographic separation was performed using an Atlantis® T3 column， and gradient elution was performed using the methanol-5 mmol/L ammonium format solution as the mobile phase. The method applied positive electrospray ionization and multiple reaction monitoring mode and was quantified with the isotope internal standard method.ResultsA good linearity was obtained with a correlation coefficient above 0.99. The average recovery levels from three different ranged from 92.3% to 94.2%， and the relative standard deviations were between 3.03% and 5.34% （n=6）. The limit of quantification was 1 μg/kg.ConclusionThis method is simple， accurate with high sensitivity and high selectivity， and suitable for the determination of vitamin D in infant formula food. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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