Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model
| Autor: | Abdullah Unlu, Muhammet Volkan Bulbul, Bircan Kolbasi Erkan, İlkay Ozdemir, Ilknur Keskin, Mustafa Sutcu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Archives of Aesthetic Plastic Surgery, Vol 29, Iss 3, Pp 129-135 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2234-0831 2288-9337 |
| DOI: | 10.14730/aaps.2022.00773 |
| Popis: | Background Since cartilage, unlike skin, does not contain vessels, it obtains nutrition by diffusion. This reduces graft viability, resulting in problems such as reductions in size, changes in shape, and resorption of the cartilage graft in the late post-graft period. This study aimed to investigate the effects of adipose-derived mesenchymal cells and conditioned medium (CM) on cartilage graft viability. Methods Dissections were performed 4 months after the injection of 0.5 mL of CM or 2×106 mesenchymal stem cells (MSCs) in 0.5 mL after grafting into a control group and two experimental groups (n=21 rabbits in total). Chondrocyte viability and type II collagen expression in the grafted areas were analyzed by hematoxylin-eosin staining and immunohistochemical methods, respectively. Results In the MSC and CM groups, chondrocyte proliferation at the graft tissue incision margin (MSC: P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|