| Autor: |
Caiting Peng, Li Wang, Yuan Liang, Li Che, Rongjing Sun, Jia Yu, Jiamin Gong, Dandan Wang, Suizhi Cheng, Qingqing Yang, Tao Jing, Zhenzhong Liu |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
The Turkish Journal of Gastroenterology, Vol 35, Iss 11, Pp 839-848 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2148-5607 |
| DOI: |
10.5152/tjg.2024.23506 |
| Popis: |
Background/Aims: N-Methyl-N’-nitroso-N-nitrosoguanidine (MNNG) is suspected to increase the risk of developing stomach cancer. Folic acid (FA) is familiar with decreasing inflammation. We expected that FA would protect against MNNG-induced gastric mucosal injury. Materials and Methods: Thirty 12-week-old SPF-grade female Sprague-Dawley (SD) rats were treated with MNNG and given different dosages of FA as an intervention measure. Quantitative polymerase chain reaction (qPCR) was used to analyze the expression of IL-1, IL-6, IL-8, IL-18, TNF-α, NLRP3, ASC, and caspase-1 genes. The enzyme-linked immunosorbent assay (ELISA) was utilized for the identification of inflammatory cytokines. Western blot was accustomed to detecting IL-1β, IL-18, and NLRP3 inflammatory vesicles in gastric tissue. Furthermore, the gastric mucosal tissues underwent histological examination. Results: Our investigation demonstrated that FA reduced MNNG-induced inflammatory factor increase by decreasing NF-κB signaling (P < .05). Furthermore, FA prevented the MNNG-induced upregulation of NLRP3 inflammasome-related genes and proteins (all P < .01). Conclusion: Our data imply that MNNG exposure stimulates the NF-κB/NLRP3 pathway, while FA suppresses it, limiting stomach mucosal inflammation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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