| Autor: |
YU Shasha, HU Hu, QIN Jie, WANG Jing |
| Jazyk: |
čínština |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Di-san junyi daxue xuebao, Vol 43, Iss 12, Pp 1140-1145 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1000-5404 |
| DOI: |
10.16016/j.1000-5404.202012233 |
| Popis: |
Objective To amplify and analyze the full-length cDNA of Anopheles dirus vitellogenin (AdVg) gene, and express and purify its functional domain. Methods Based on the cDNA fragments of AdVg gene cloned earlier, the full-length sequence was obtained through rapid amplification of cDNA ends (RACE). The sequences of AdVg cDNA fragment, 5' end and 3' end were corrected and assembled to obtain a complete AdVg cDNA full-length sequence by Vector NTI software. Then the full-length sequence of AdVg was used to predict the functional domain. Finally, recombinant plasmid pet28A (+) was used to express and purify the AdVg functional domain. Results The full-length cDNA sequence of the AdVg gene was successfully obtained, including a 117 bp 5' UTR sequence, a 192 bp 3'UTR sequence, and a 6 186 bp open reading frame (ORF) sequence. Meanwhile, the analysis showed that the AdVg protein mainly contained 3 structural domains, including Vit N, DUF1943 and vWD. Then we successfully expressed and purified the functional domain AdVg Vit N by constructing a recombinant plasmid. Conclusion The full-length cDNA of AdVg is successfully cloned, and the recombinant protein of its functional domain Vit N is obtained in this study. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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