An Efficient Method of Extracting PCR Amplifiable DNA from Human Skeletal Fragments and Teeth Specimens.
| Autor: | Abdullahi Daudu Zagga, Somaia Muhammad Ismail, Aziz Abdo Tadros, Hamidu Ahmed |
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| Jazyk: | English<br />Turkish |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Çukurova Üniversitesi Tıp Fakültesi Dergisi, Vol 39, Iss 4, Pp 712-721 (2014) |
| Druh dokumentu: | article |
| ISSN: | 0250-5150 80285414 |
| Popis: | Purpose:Extraction and successful PCR amplification of DNA from humanremains in historical and forensic cases have great importance. The purpose of this study is to demonstrate the efficiency of a method of DNA extraction and PCR amplification of embalmed dried human cadaveric skeletal fragments and teeth specimens from Sokoto, Northwestern Nigeria. Materials and Methods: The efficiency of a method of DNA extraction and PCR amplification was tested on thirteen (13) embalmed dried human cadaveric skeletal fragments and nine (9) teeth specimens from Sokoto, Northwestern Nigeria were used in the study. Results: Of the 13 embalmed dried human cadaveric skeletal fragments, 12 (92.3%) samples amplified with apparent bands. For the 9 embalmed dried human teeth specimens, 7 (78%) samples amplified with apparent bands. Conclusion: The study has shown that our method of DNA extraction and PCR amplification was efficient on embalmed dried human cadaveric skeletal fragments and teeth specimens, from Sokoto, Northwestern Nigeria. [Cukurova Med J 2014; 39(4.000): 712-721] |
| Databáze: | Directory of Open Access Journals |
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