Autor: |
C.S. Metsopkeng, M.E. Nougang, P.A. Nana, A. Tamsa Arfao, P. Ngo Bahebeck, C. Lontsi Djimeli, J.S. Eheth, O.V. Noah Ewoti, L.M. Moungang, G.A. Agbor, F. Perrière, T. Sime-Ngando, M. Nola |
Jazyk: |
angličtina |
Rok vydání: |
2020 |
Předmět: |
|
Zdroj: |
Scientific African, Vol 10, Iss , Pp e00549- (2020) |
Druh dokumentu: |
article |
ISSN: |
2468-2276 |
DOI: |
10.1016/j.sciaf.2020.e00549 |
Popis: |
The present study assessed the abiotic factors of Moringa stenopetala (M. stenopetala) extracts against Staphyloccoccus aureus in aquatic microcosm. Bacterial survival was assessed using colony forming unit (CFU) method on culture agar medium at different concentration levels (0.5 to 2 g/L). The incubation duration varied from 3 to 24 hours, and the temperatures were between 7°C and 44°C. Phytochemical screening showed that most of the constituents obtained from the extracts are the alkaloids, flavonoids and the tannins. It was observed that increase in extract concentration significantly inhibited the number of CFU obtained. Compared to the control {9.27 Log(CFU/100 mL)}, cells abundance from different concentrations of leaves extracts fluctuated between 4.74 and 0 {Log(CFU/100 mL)} after 3h incubation; 5.46 and 0 {Log(CFU/100 mL)} after 6h; 4.75 and 0 {Log(CFU̸/100 mL)} after 9h, and 4.85 and 0 {Log(CFU/100 mL)} after 24h incubation. As for root extracts, the abundance were between 4.80 and 0 {Log(CFU/100 mL)} under 7°C incubation; 5.03-5.23 and 0 {Log(CFU/100 mL)} under 23°C and 37°C, and 4.66 and 0 Log (CFU/100 mL) under 44°C incubation when considering the whole extract concentrations used. Incubation temperature and extracts concentration significantly impacted bacterial abundances. When the decoction extracts of roots were used, the amount of S. aureus cells inhibited was registered after 3-24 hours incubation. The Pearson correlation coefficient in most cases, revealed an inversely proportional relationship between the plant extracts and S. aureus cultured cells in a dose-dependent manner (P≤0.05). The use of M. stenopetala extracts could constitute an alternative in the treatment of water intended for human consumption. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
|