Removal of sequencing adapter contamination improves microbial genome databases

Autor:	Andrew H. Moeller, Brian A. Dillard, Samantha L. Goldman, Madalena V. F. Real, Daniel D. Sprockett
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	Bacterial genomes Metagenome Microbiota Symbiosis Microbial ecology Biotechnology TP248.13-248.65 Genetics QH426-470
Zdroj:	BMC Genomics, Vol 25, Iss 1, Pp 1-6 (2024)
Druh dokumentu:	article
ISSN:	1471-2164
DOI:	10.1186/s12864-024-10956-1
Popis:	Abstract Advances in assembling microbial genomes have led to growth of reference genome databases, which have been transformative for applied and basic microbiome research. Here we show that published microbial genome databases from humans, mice, cows, pigs, fish, honeybees, and marine environments contain significant sequencing-adapter contamination that systematically reduces assembly accuracy and contiguousness. By removing the adapter-contaminated ends of contiguous sequences and reassembling MGnify reference genomes, we improve the quality of assemblies in these databases.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/bb791503e7804d289d2e3202cacd083c Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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