Development of an ELISA-based kit for the on-farm determination of progesterone in milk
| Autor: | R. Simersky, J. Swaczynova, D.A. Morris, M. Franek, M. Strnad |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Veterinární Medicína, Vol 52, Iss 1, Pp 19-28 (2007) |
| Druh dokumentu: | article |
| ISSN: | 0375-8427 1805-9392 |
| DOI: | 10.17221/2009-VETMED |
| Popis: | Effective reproductive management is a crucial concern in the dairy industry and missed oestrus and late recognition of unsuccessful insemination can lead to substantial financial loss. Progesterone levels change in a predictable manner with progress of the oestrus cycle and with the onset of pregnancy. We report here the development of a simple ELISA test for the measurement of progesterone levels in bovine milk. The method is based on the use of polyclonal antibodies raised against 11α-hydroxyprogesterone-hemisuccinate and competition for antibody binding sites between free progesterone and horseradish peroxidase-labelled progesterone. Binding is quantified by means of the colour reaction between horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine. The method has proved to be a cost effective, sensitive and easy to perform alternative to currently available methods of oestrus and pregnancy detection. The practical feasibility of the method has been proven under dairy farm conditions. |
| Databáze: | Directory of Open Access Journals |
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