TAPE-seq is a cell-based method for predicting genome-wide off-target effects of prime editor

Autor: Jeonghun Kwon, Minyoung Kim, Seungmin Bae, Anna Jo, Youngho Kim, Jungjoon K. Lee
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-022-35743-y
Popis: Methods to predict genome-wide off-target activities of prime editors (PEs) are currently lacking. Here the authors report a cell-based assay, TAgmentation of Prime Editor sequencing (TAPE-seq), that provides genome-wide off-target candidates for PEs.
Databáze: Directory of Open Access Journals