Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis

Autor: Taketoshi Mizutani, Samuel Yaw Aboagye, Aya Ishizaka, Theophillus Afum, Gloria Ivy Mensah, Adwoa Asante-Poku, Diana Asema Asandem, Prince Kofi Parbie, Christopher Zaab-Yen Abana, Dennis Kushitor, Evelyn Yayra Bonney, Motoi Adachi, Hiroki Hori, Koichi Ishikawa, Tetsuro Matano, Kiyosu Taniguchi, David Opare, Doris Arhin, Franklin Asiedu-Bekoe, William Kwabena Ampofo, Dorothy Yeboah-Manu, Kwadwo Ansah Koram, Abraham Kwabena Anang, Hiroshi Kiyono
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-021-93345-y
Popis: Abstract Acute gastroenteritis associated with diarrhea is considered a serious disease in Africa and South Asia. In this study, we examined the trends in the causative pathogens of diarrhea and the corresponding gut microbiota in Ghana using microbiome analysis performed on diarrheic stools via 16S rRNA sequencing. In total, 80 patients with diarrhea and 34 healthy adults as controls, from 2017 to 2018, were enrolled in the study. Among the patients with diarrhea, 39 were norovirus-positive and 18 were rotavirus-positive. The analysis of species richness (Chao1) was lower in patients with diarrhea than that in controls. Beta-diversity analysis revealed significant differences between the two groups. Several diarrhea-related pathogens (e.g., Escherichia-Shigella, Klebsiella and Campylobacter) were detected in patients with diarrhea. Furthermore, co-infection with these pathogens and enteroviruses (e.g., norovirus and rotavirus) was observed in several cases. Levels of both Erysipelotrichaceae and Staphylococcaceae family markedly differed between norovirus-positive and -negative diarrheic stools, and the 10 predicted metabolic pathways, including the carbohydrate metabolism pathway, showed significant differences between rotavirus-positive patients with diarrhea and controls. This comparative study of diarrheal pathogens in Ghana revealed specific trends in the gut microbiota signature associated with diarrhea and that pathogen-dependent dysbiosis occurred in viral gastroenteritis.
Databáze: Directory of Open Access Journals
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