Correlation of cytokine profile mononuclear and endothelial progenitor cells, obtained in the course of the mobilization of granulocyte colony-stimulating factor in patients with chronic heart failure

Autor: О. В. Повещенко, Н. А. Бондаренко, А. П. Лыков, И. И. Ким, М. А. Суровцева, А. Ф. Повещенко, Е. А. Покушалов, А. Б. Романов, А. М. Караськов, В. И. Коненков
Jazyk: English<br />Russian
Rok vydání: 2016
Předmět:
Zdroj: Патология кровообращения и кардиохирургия, Vol 19, Iss 4-2, Pp 55-61 (2016)
Druh dokumentu: article
ISSN: 1681-3472
2500-3119
DOI: 10.21688/1681-3472-2015-4-2-55-61
Popis: Objective: The aim of the study was to evaluate the ability of cytokine peripheral blood mononuclear cells and its correlation with the release of endothelial progenitor cells after mobilization of G-CSF in patients with chronic heart failure.Methods: Mononuclear cells were obtained from peripheral blood of 35 patients with chronic heart failure before and after mobilization procedures G-CSF (granulocyte colony stimulating factor). Spectrum of cytokine production and growth factors mononuclear cells was evaluated by ELISA in spontaneous conditions and upon stimulation of cells with concanavalin A, lipopolysaccharide, G-CSF, erythropoietin. Results: A statistically significant increase in the spontaneous production of IL-18, VEGF, Epo and reducing TNF-α production and G-CSF mononuclear cells after mobilization procedures G-CSF. A statistically significant increase in mononuclear cell production of IL-18, VEGF, and G-CSF in response to mitogenic stimulation (Con A) and decrease in production of IL-8 after mobilization procedures G-CSF. In response to an antigenic stimulus (LPS) mononuclear cells were enriched with endothelial progenitor cells from patients with chronic heart failure responded statistically significant increase in the production of IL-18 and G-CSF, and decreased production - TNF-α, as compared to similar products of cytokines and growth factors prior to the procedure to mobilize G-CSF. Proangiogenic cytokines G-CSF or Epo result in a statistically significant increase in the production TNF-α, IL-10, VEGF, and G-CSF mononuclear cells enriched endothelial progenitor cells in patients with chronic heart failure.Conclusion: Peripheral blood mononuclear cells, endothelial progenitor cells enriched after mobilization of G-CSF, in patients with chronic heart failure produce cytokines and growth factors with proangiogenic effect. Thus, endothelial progenitor cells contribute to the production of cytokines and growth factors such as TNF-α, IL-18, IL-10, Epo, VEGF. Mononuclear cells were obtained after mobilization of G-CSF, can be used to treat chronic heart failure.
Databáze: Directory of Open Access Journals
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