RESULTS OF SSR ANALYSIS, PROPERTIES OF PLANT MORPHOLOGY AND BIOCHEMICAL COMPOSITION OF CHINESE BROCCOLI – A NEW VEGETABLE CROP FOR RUSSIA

Autor: Y. V. Fotev, A. M. Artemyeva, D. A. Fateev, N. B. Naumova, G. A. Bugrovskaya, V. P. Belousova, T. A. Kukushkina
Jazyk: English<br />Russian
Rok vydání: 2018
Předmět:
Zdroj: Овощи России, Vol 0, Iss 1, Pp 12-19 (2018)
Druh dokumentu: article
ISSN: 2072-9146
2618-7132
DOI: 10.18619/2072-9146-2018-1-12-19
Popis: The results of estimation of growth and development parameters, SSR analysis, electron microscopic examination of the surface of pollen and seeds, biochemical composition (ascorbic acid, carotenoids, carotenes, macro- and microelements content in the stem part of plant) of 8 Chinese broccoli, Brassica oleracea var. alboglabra samples grown in the unheated greenhouse of the Central Siberian Botanical Garden SB RAS, Novosibirsk (54°49′33″ N, 83°06′34″ E) and phy- totron of N. I. Vavilov Institute of Plant Genetic Resources (VIR) have been presented. Through the study, the large surface cellularity of B.oleracea var. alboglabra (cv. “Siji Xianggu jie lan” [“SX”]) seeds was similar with seed coat surface of B.oleraceae var. capitata f. alba cv. "Slava". A scanning electron microscopic study of Chinese broccoli pollen (cv. “SX”) revealed the formation of colpate pollen with ellipsoidal shape with a size of 32.9 ± 0.76 x 17.8 ± 0.42 μm, characterized by foveolate ornamentation with a length-to-diameter ratio – 1.85.The duration of the period from emergence stage to thickening of a stalk and flower-bud formation of cv. “SX” continued for 49-54 days. Сlose correlation was established between the largest diameter of the stem and the length of the leaf petiole (r=0,87, p < 0,001) and between the length of the leaf petiole and the weight of the stem (r=0,77, p < 0,001). As a result of molecular screening of Chinese broccoli using 5 SSR markers (Na10D09, Ol12F02, Ra2E12, BC 7 и BC 65) the greatest polymorphism was in the test with the BC 7 marker - five alleles from 160 to 295 bp in size. In the “SX” variety single flowering (10%) occurred on 59 ... 63 day and mass flowering (75%) – on 65 ... 68 day. The stem part of B. oleracea var. alboglabra is a fairly good source of vitamin C, accumulating as much as 32-46 mg/100 g, and also increased concentration of K, Ca, Mg and Cu. At the end of the season the fruits, together with the phytomass of stems are well-seasoned for 3-4 weeks forming viable seeds with germination above 85% corresponding to the Russian state standard (GOST 32592-2013) for white cabbage seeds.
Databáze: Directory of Open Access Journals
Externí odkaz: