Insulin Stimulation of Protein Synthesis and mTOR Signaling in Chick Myotube Cultures
| Autor: | Kazuki Nakashima, Aiko Ishida, Saki Shimamoto, Daichi Ijiri, Akira Ohtsuka |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | The Journal of Poultry Science, Vol 57, Iss 3, Pp 205-209 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1346-7395 1349-0486 |
| DOI: | 10.2141/jpsa.0190082 |
| Popis: | Insulin stimulates protein synthesis in skeletal muscles. Protein synthesis is controlled by the mechanistic target of rapamycin (mTOR) signaling in skeletal muscles. This study was conducted to investigate the effect of insulin on protein synthesis and mTOR signaling in chick myotube cultures. Chick myotubes were incubated with insulin (1 µg/ml) for 1 h. Protein synthesis, measured using the surface sensing of translation method, was significantly increased by insulin. The phosphorylation of AKT (Thr308 and Ser473), p70 ribosomal S6 kinase 1 (S6K1, Thr389), S6 ribosomal protein (Ser235/236), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1, Thr37/46) was also significantly increased by insulin. These results suggest that insulin stimulates protein synthesis via mTOR signaling (phosphorylation of AKT, S6K1, S6 ribosomal protein, and 4E-BP1) in chick myotube cultures. |
| Databáze: | Directory of Open Access Journals |
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