| Autor: |
María Buendía-Abad, Mariano Higes, Raquel Martín-Hernández, Laura Barrios, Aranzazu Meana, Alberto Fernández Fernández, Antonio Osuna, Luis Miguel De Pablos |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
International Journal for Parasitology: Parasites and Wildlife, Vol 14, Iss , Pp 68-74 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2213-2244 |
| DOI: |
10.1016/j.ijppaw.2020.12.003 |
| Popis: |
The impact of trypanosomatid parasites on honeybee health may represent a major threat to bee colonies worldwide. However, few axenic isolates have been generated to date and with no details on cell culture passages, a parameter that could influence parasite virulence. To address this question, a trypanosomatid isolation protocol was developed and a new strain was obtained, named L. passim C1. Using experimental infection of worker honeybees, we compared the virulence and mortality rates of the ATCC PRA-403 reference strain and C1 strain, the latter showing higher virulence from 10 days post-infection onward. This study highlights the impact of cell culture passages on the pathogenicity of L. passim in honeybees, providing new evidence of its negative effects on honeybee health. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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