A rapid test for detection of tribenuron-methyl resistance in sunflower
| Autor: | Dimitrijević Aleksandra, Imerovski Ivana, Miladinović Dragana, Jocić Siniša, Cvejić Sandra, Malidža Goran, Kovačević Branislav, Šurlan-Momirović Gordana |
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| Jazyk: | English<br />Serbian |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Ratarstvo i Povrtarstvo, Vol 53, Iss 1, Pp 1-8 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1821-3944 2217-8392 |
| DOI: | 10.5937/ratpov53-8412 |
| Popis: | A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively. |
| Databáze: | Directory of Open Access Journals |
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