Micro‐Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells

Autor: Steven Vermeulen, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. van derVeer, Dieter Deforce, Maarten Dhaenens, Jan de Boer
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Advanced Science, Vol 10, Iss 1, Pp n/a-n/a (2023)
Druh dokumentu: article
ISSN: 2198-3844
97722448
DOI: 10.1002/advs.202203880
Popis: Abstract Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression and cell identity, surface topography can control cell phenotype. This study provides fundamental insights into how surface topography influences nuclear morphology, histone modifications, and expression of histone‐associated proteins through advanced histone mass spectrometry and microarray analysis. The authors find that nuclear confinement is associated with a loss of histone acetylation and nucleoli abundance, while pathway analysis reveals a substantial reduction in gene expression associated with chromosome organization. In light of previous observations where the authors found a decrease in proliferation and metabolism induced by micro‐topographies, they connect these findings with a quiescent phenotype in mesenchymal stem cells, as further shown by a reduction of ribosomal proteins and the maintenance of multipotency on micro‐topographies after long‐term culture conditions. Also, this influence of micro‐topographies on nuclear morphology and proliferation is reversible, as shown by a return of proliferation when re‐cultured on a flat surface. The findings provide novel insights into how biophysical signaling influences the epigenetic landscape and subsequent cellular phenotype.
Databáze: Directory of Open Access Journals
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