| Autor: |
M. Subbulakshmi, Sugapriya Dhanasekaran, S. Abirami, M. Kannan, R. Palaniappan, Divya Venugopal |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Food Science and Human Wellness, Vol 10, Iss 4, Pp 452-459 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2213-4530 |
| DOI: |
10.1016/j.fshw.2021.04.007 |
| Popis: |
Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects. This study identified a unique isoform of 18S rRNA gene (864 bp) from a novel wild mushroom (SMK-1) (GenBank accession number: SUB3267363). Thin layer chromatographic (TLC) profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and flavonoid fractions with retention factor (Rf) values of 0.955 and 0.927 respectively. The GC/MS chromatograms of the SMK-1 methanolic extract identified the main bioactive compound was phenol, 5-methyl-2-(1-methylethyl) (74.00%) (thymol). The radical scavenging activity for the flavonoid fraction was greater than the phenolic fractions (Rf–phenolics fractions > Rf–flavonoid fractions) with the antioxidant activity more than that of standard ascorbic acid. Also, the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5 μg/mL to 80 μg/mL in a dose-dependent manner. This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefits. The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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