Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina.
| Autor: | L Pinheiro, S Franco, I S Adagu, R Rosa, V E Rosário, D C Warhurst |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Acta Médica Portuguesa, Vol 16, Iss 4 (2003) |
| Druh dokumentu: | article |
| ISSN: | 0870-399X 1646-0758 |
| DOI: | 10.20344/amp.1191 |
| Popis: | Isolates of Plasmodium falciparum from three areas of West Africa were recovered from cryopreservation and their chloroquine-sensitivity were determined in vitro. Of the 90 samples studied, 60 were from Guinea-Bissau (30Resistant/30Sensitive), 15 were from S. Tomé and Príncipe (11Resistant/4Sensitive) and 15 were from Angola (11Resistant/4Sensitive). All the isolates were sensitive to mefloquine. Using the polymerase chain reaction/restriction fragment length polymorphism technique (PCR/RFLP) it was possible to detect two mutations in the pfmdr1 gene, often associated with chloroquine-resistance. 66% of the samples from Guiné-Bissau showed a correlation with chloroquine-resistance while 73% of the samples from São Tomé and Angola altogether had the 86Tyr mutation. The present study on West African isolates and clones showed, for the first time, the presence of a double point mutation in the pfmdr1 gene one being found, up to now, only in South America isolates of Plasmodium falciparum. |
| Databáze: | Directory of Open Access Journals |
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