Bioprospection of ligninolytic enzymes from marine origin filamentous fungi
| Autor: | CAMILA P. DANTAS, JOÃO PEDRO D. PINCHEMEL, GISELE M. DE JESUS, MILENA B. PIMENTEL, OLÍVIA MARIA C. OLIVEIRA, ANTÔNIO FERNANDO S. QUEIROZ, DANUSIA F. LIMA |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Anais da Academia Brasileira de Ciências, Vol 93, Iss suppl 3 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1678-2690 0001-3765 |
| DOI: | 10.1590/0001-3765202120210296 |
| Popis: | Abstract Fungi are excellent producers of extracellular enzymes. Therefore, the present study aimed to investigate the screening of marine fungi, which are laccase and manganese peroxidase potential producers, in solid fermentation for future applications in bioremediation processes of contaminated sites. For this purpose, two-level factorial planning was adopted, using time (6 and 15 days) and the absence or presence of oil (0 and 1%) as factors. The semi-quantitative evaluation was carried out by calculating radial growth, enzyme activity and enzyme index by measuring phenol red or syringaldazine oxidation halo. The results showed that all the studied strains showed a positive result for manganese peroxidase production, with an enzymatic activity in solid medium less than 0.61, indicating a strongly positive activity. Through the enzyme index, the study also showed prominence for Penicillium sp. strains, with values > 2. The enzyme index increase in oil presence and the inexpressive use of the genera studied for ligninolytic enzymes production from crude oil demonstrated these data importance for fermentative processes optimization. Considering the ability of these strains to develop into recalcitrant compounds and the potential for manganese peroxidase production, they are indicated for exploitation in various bioremediation technologies, as well as other biotechnological applications. |
| Databáze: | Directory of Open Access Journals |
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