| Autor: |
Pauline Jéhannet, Arjan P. Palstra, Ignacio Giménez Nebot, Henk Schipper, William Swinkels, Leon T. N. Heinsbroek, Hans Komen |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Fishes, Vol 8, Iss 3, p 123 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2410-3888 |
| DOI: |
10.3390/fishes8030123 |
| Popis: |
Commonly, female European eels are injected weekly with pituitary extract (PE) from carp (CPE) or salmon (SPE) to induce sexual maturation. However, a PE is a mixture of gonadotropins and other hormones that are not specific for eel and rapidly cleared from circulation. The aim of this study was therefore to test the effects of highly stable eel-specific recombinant gonadotropins (rGTHs) on oocyte development in vitro and in vivo in European eels. For the in vitro trial, the dose–effect responses of maturing eel oocytes on CPE and recombinant luteinizing hormone (rLH) were studied before and after 12 and 18 h of incubation. For the in vivo experiment, sexual maturation was stimulated by treatment with (i) CPE, (ii) recombinant follicle-stimulating hormone (rFSH) followed by CPE and (iii) rFSH followed by rLH. For the in vitro experiment, the expression of the nuclear progestin receptor 2 (pgr2) was induced by rLH, implying that rLH was preparing the oocyte for ovulation. For the in vivo experiment, the females treated with rGTHs had high gonadosomatic index (GSI) values (rFSH-CPE: 75, 77; rFSH-rLH: 80) in comparison with the females injected with CPE (50–60), suggesting that rFSH strongly induced vitellogenic growth. Larvae were produced for all treatment groups and for the first time by rGTH treatment alone but dose and timing still need optimization. |
| Databáze: |
Directory of Open Access Journals |
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