| Autor: |
Wenberger Lanza Daniel de Figueiredo, Eraldo Ferreira Lopes, Deborah Laredo Jezini, Lorena Naciff Marçal, Enedina Nogueira de Assunção, Paulo Rodrigo Ribeiro Rodrigues, Adolfo José da Mota, Diego Monteiro de Carvalho, Spartaco Astolfi Filho, João Bosco Lopes Botelho |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
PLoS ONE, Vol 17, Iss 5, p e0268354 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0268354 |
| Popis: |
IntroductionThe goiter, a neglected heterogeneous molecular disease, remains a major indication for thyroidectomies in its endemic regions.ObjectivesThis study analyzed differential gene expression in surgical specimens diagnosed with multi nodular and compared the data to that of thyroid tissue without multinodular goiter from patients undergoing thyroidectomy in Manaus-AM, Brazil using RNA-seq technology.MethodologyThe transcriptome information of the surgical specimen fragments with and without multinodular goiter was accessed by Illumina HiSeq 2000 New Generation Sequencing (NGS) using the RNA-seq NEBNext® Ultra™ RNA Library Prep Kit for Illumina®-#E7530L protocol and differential gene expression analysis.ResultsDifferences were found between the gene expression profiles of the diseased tissues and those of the healthy control tissues; at least 70 genes were differentially expressed. The HOTS gene was expressed only in multinodular goiter tissues (p < 0.05).ConclusionThese results demonstrate that the gene expression profile of multinodular goiter is pro-tumoral and that HOTS can play a central role in multinodular goiter development. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
