| Autor: |
Angeliki Mavroidi, Anna Katsiaflaka, Efthymia Petinaki, Elisavet Froukala, Dimitrios Papadopoulos, Georgia Vrioni, Athanasios Tsakris |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Journal of Global Antimicrobial Resistance, Vol 37, Iss , Pp 185-189 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2213-7165 |
| DOI: |
10.1016/j.jgar.2024.03.016 |
| Popis: |
ABSTRACT: Objectives: Streptococcus pyogenes causes superficial infections but can also cause deep-seated infections and toxin-mediated diseases. In the present study, phylogenetic and in silico prediction analyses were performed on an antimicrobial resistant M1UK S. pyogenes strain causing severe clinical manifestations during the current surge of invasive group A Streptococcus (iGAS) disease. Methods: A 40-year-old patient was admitted to the hospital with fever, chest pain and fatigue. Based on the clinical and laboratory findings, a diagnosis of sepsis with disseminated intravascular coagulation, community-acquired pneumonia, pleural empyema and streptococcal toxic shock syndrome was made. Microbial identification was performed by multiplex PCR and conventional culturing. Furthermore, antimicrobial susceptibility testing, whole genome sequencing, phylogenomic analysis and in silico prediction analysis of antimicrobial resistance genes and virulence factors were performed. Results: S. pyogenes isolates were detected in pleural fluid and sputum of the patient. Both isolates belonged to the M1UK lineage of the emm1/ST28 clone, being closely related with an M1UK GAS strain from Australia. They exhibited resistance to erythromycin and clindamycin and susceptibility-increased exposure to levofloxacin and carried genes encoding for protein homologues of antibiotic efflux pumps. Moreover, several virulence factors, and a previously described single-nucleotide polymorphism in the 5′ transcriptional leader sequence of the ssrA gene, which enhances expression of SpeA, were detected. Conclusions: The present antimicrobial-resistant M1UK S. pyogenes strain represents the first report of this emerging lineage associated with such manifestations of iGAS disease. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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