Análise quantitativa das células das ilhotas pancreáticas em ratos sob efeito de aloxana
| Autor: | Marcus Aurelho Lima, Lilian Margareth Biagioni Lima, Danielle Patrícia Castanheira Rita, Fábio Castilho Navarro, Ranaia Santana Tatsukawa, Gilberto Araújo Pereira, Luiz Carlos Reis, Marta Elena Araújo Abreu, Maria de Fátima Borges |
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| Jazyk: | portugalština |
| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Medicina, Vol 34, Iss 3/4 (2001) |
| Druh dokumentu: | article |
| ISSN: | 2176-7262 0076-6046 |
| DOI: | 10.11606/issn.2176-7262.v34i3/4p308-314 |
| Popis: | In the present study, we verified the influence of alloxan over the cells of pancreatic islet of rats, performing quantitative analysis of a, b and d cells through immunohistochemical study. Twenty male rats (Rattus norvegiccus) were used of Wistar line, disposed in 2 groups of 10 rats on each, being a control group and an experimental one. After fasting of 36 hours, 40 ml/Kg of monohydrated of alloxan was administrated by intravenous via and about 30 minutes after, the animal was offered food. At the end of 10 days of observation, animals were sacrificed. Samples of different regions of pancreas were selected and undergone to a histological process and stained with hematoxilin-eosin. To the immunohistochemical study, anti-insulin, anti-glucagon and anti-somatostatin antibodies were used. Quantitative analysis was performed in eight most representative pancreatic islets of each animal. Results were considered significative when p |
| Databáze: | Directory of Open Access Journals |
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