Gas-liquid chromatography of ethyl ester artifacts formed during the preparation of fatty acid methyl esters

Autor: A R Johnson, A C Fogerty, R L Hood, S Kozuharov, G L Ford
Jazyk: angličtina
Rok vydání: 1976
Předmět:
Zdroj: Journal of Lipid Research, Vol 17, Iss 4, Pp 431-432 (1976)
Druh dokumentu: article
ISSN: 0022-2275
DOI: 10.1016/S0022-2275(20)34930-0
Popis: Ethanol is typically used as a stabilizer in chloroform. Failure to remove this ethanol from the chloroform used in the extraction of lipids leads subsequently to the formation of ethyl ester artifacts during the preparation of methyl esters by a commonly employed transesterification procedure. Depending on the conditions and phases used during gas-liquid chromatography, the ethyl esters may be resolved from the corresponding methyl esters. The resulting chromatograms contain extraneous peaks and may be incorrectly identified.
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