| Autor: |
Haotian Deng, Yanwen Kong, Jinyan Zhu, Xinyao Jiao, Yuqi Tong, Meizhi Wan, Yang Zhao, Sixu Lin, Yan Ma, Xianjun Meng |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Current Research in Food Science, Vol 5, Iss , Pp 1559-1569 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2665-9271 |
| DOI: |
10.1016/j.crfs.2022.09.017 |
| Popis: |
Aronia melanocarpa anthocyanins (AMAs), as a natural plant extract, can control pathogens and are attracting increasing attention. However, at the proteomic level, the antibacterial mechanism of AMAs against Escherichia coli O157: H7 remains unclear. In this study, the tandem mass tag (TMT) quantitative proteomics was used to elucidate the potential antibacterial mechanisms of AMAs against E. coli O157: H7 cells. Ultrastructural observation and reactive oxygen species (ROS) levels detection showed that AMAs destroyed the integrity of E. coli O157: H7 cell membrane, led to the aggregation of contents and caused the increase of intracellular ROS level. TMT-based proteomic analysis suggested that AMAs can enter cells through mechanosensitive channels and inhibit the activity of heat shock proteins; disturb the metabolism of carbohydrates, amino acids and lipids in cells. The results of this study provide a reference for the further development of plant-derived antimicrobial agents. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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