| Autor: |
Ryan A. Kelley, Holly Y. Chen, Anand Swaroop, Tiansen Li |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 1, Iss 1, Pp 100033- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100033 |
| Popis: |
Summary: Human pluripotent stem cells (PSCs) can be differentiated into retinal organoids with proper neural layer organization, yet the protocols are technically challenging and time consuming. We have modified a widely used differentiation protocol by switching all-trans retinoic acid with 9-cis retinal to accelerate photoreceptor differentiation and improve morphogenesis. In this report, we provide a detailed and improved protocol to generate retinal organoids from human pluripotent stem cells.For complete details on the use and execution of this protocol, please refer to Kaya et al. (2019). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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