Development of 17 microsatellite markers in the federally endangered species Liatris helleri (Asteraceae)

Autor:	Logan C. Clark, Morgan R. Gaglianese‐Woody, Matt C. Estep
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Asteraceae endangered species Liatris helleri perennial herb Southern Appalachians species boundaries Biology (General) QH301-705.5 Botany QK1-989
Zdroj:	Applications in Plant Sciences, Vol 7, Iss 7, Pp n/a-n/a (2019)
Druh dokumentu:	article
ISSN:	2168-0450
DOI:	10.1002/aps3.1250
Popis:	Premise Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus. Methods and Results Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The primers amplified motifs from tri‐ to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross‐amplification in L. aspera, L. microcephala, and L. pycnostachya. Conclusions The developed primers for L. helleri serve as a novel genetic tool for future investigations in this genus, allowing for more explicit species delineation as well as population genetic analyses.
Databáze:	Directory of Open Access Journals
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