Protocol for measuring cell cycle Zn2+ dynamics using a FRET-based biosensor
| Autor: | Samuel E. Holtzen, Ananya Rakshit, Amy E. Palmer |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | STAR Protocols, Vol 5, Iss 2, Pp 103122- (2024) |
| Druh dokumentu: | article |
| ISSN: | 2666-1667 32141408 |
| DOI: | 10.1016/j.xpro.2024.103122 |
| Popis: | Summary: The exchangeable Zn2+ pool in cells is not static but responds to perturbations as well as fluctuates naturally through the cell cycle. Here, we present a protocol to carry out long-term live-cell imaging of cells expressing a cytosolic Zn2+ sensor. We then describe how to track cells using the published pipeline EllipTrack and how to analyze the single-cell traces to determine changes in labile Zn2+ in response to perturbation.For complete details on the use and execution of this protocol, please refer to Rakshit and Holtzen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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