Multinucleation in the Two-Cell Stage Embryo Matters
| Autor: | Leah LAZZARO, Martin HEALEY, Tiki OSIANLIS |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Fertility & Reproduction, Vol 04, Iss 03n04, Pp 167-167 (2022) |
| Druh dokumentu: | article |
| ISSN: | 26613182 2661-3174 2661-3182 |
| DOI: | 10.1142/S2661318222740796 |
| Popis: | Background: Blastomere multinucleation (MN), defined as the presence of more than one nucleus per cell, in the cleavage-stage embryo, is a well-documented phenomenon1. This nuclear anomaly has become more visible with the introduction of time-lapse incubation and an understanding of the potential outcomes of these embryos is necessary to inform decision-making and manage patient expectations. Aim: To determine rates of blastocyst utilisation and euploidy for embryos with MN at the two-cell stage (MN2) in comparison to mononucleated and binucleated embryos. Method: A retrospective analysis was performed at a single IVF unit, of embryos with known two-cell nucleation status from October 2019 to March 2021. Blastomere nucleation was assessed, via time-lapse monitoring (EmbryoScope; UnisenseFertiliTech), and categorised as mononucleate, binucleate or MN in all fertilised oocytes. Blastocyst utilisation rates (number of embryos frozen+transferred/number of oocytes fertilised) were compared together with euploidy rates in embryos that had preimplantation genetic testing for aneuploidy. Outcomes were modelled using multilevel logistic regression to allow for lack of independence between embryos when grouped by patient and cycle, and adjusted for patient age. Results: Of the 4388 embryos included in this study, 68% were mononucleated, 14% binucleated and 18% MN. The likelihood of embryos becoming usable blastocysts based on nucleation status at the two-cell stage for mononucleated, binucleated and MN were 63%, 55%(p |
| Databáze: | Directory of Open Access Journals |
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