| Autor: |
Artem Nemudryi, Anna Nemudraia, Tanner Wiegand, Kevin Surya, Murat Buyukyoruk, Calvin Cicha, Karl K. Vanderwood, Royce Wilkinson, Blake Wiedenheft |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Cell Reports Medicine, Vol 1, Iss 6, Pp 100098- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2666-3791 |
| DOI: |
10.1016/j.xcrm.2020.100098 |
| Popis: |
Summary: SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here, we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 74-day time course. We show that changes in SARS-CoV-2 RNA concentrations follow symptom onset gathered by retrospective interview of patients but precedes clinical test results. In addition, we determine a nearly complete (98.5%) SARS-CoV-2 genome sequence from wastewater and use phylogenetic analysis to infer viral ancestry. Collectively, this work demonstrates how wastewater can be used as a proxy to monitor viral prevalence in the community and how genome sequencing can be used for genotyping viral strains circulating in a community. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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