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The effects of copper oxide nanoparticles (CuONPs) on germinating seedlings of green pea (Pisum sativum L.) were studied. The seedlings were grown in a half-strength Murashige and Skoog semisolid medium containing 0, 50, 100, 200, 400, and 500 mg dm-3 CuONPs for 14 d under controlled growth chamber conditions. Exposures to 100, 200, 400, and 500 mg dm-3 CuONPs significantly reduced plant growth (shoot and root lengths) and increased reactive oxygen species (ROS) generation and lipid peroxidation. Gene expression study using real-time polymerase chain reaction showed no significant change in the expression of genes coding CuZn-superoxide dismutase (CuZnSOD), catalase (CAT), and ascorbate peroxidase (APX) in shoots. However in roots, a significant increase in the expression of the CuZnSOD gene was observed under the exposures to 100, 200, 400, and 500 mg dm-3 CuONPs, in the expression of the CAT gene under 100 and 200 mg dm-3 CuONPs, and in the expression of APX under 200 and 400 mg dm-3 CuONPs. |
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