The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury

Autor: Ugur Aksu, Onur M. Yaman, Ibrahim Guner, Gulcan Guntas, Fuat Sonmez, Gamze Tanriverdi, Mediha Eser, Aris Cakiris, Sibel Akyol, İsmail Seçkin, Hafize Uzun, Nermin Yelmen, Gulderen Sahin
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Investigative Surgery, Vol 34, Iss 6, Pp 601-609 (2021)
Druh dokumentu: article
ISSN: 0894-1939
1521-0553
08941939
DOI: 10.1080/08941939.2019.1672841
Popis: Background Despite the progress in the treatment of acute kidney injury (AKI), current curative approaches fail to provide adequate treatment. In this study, we aimed to investigate the possible protective effects of thymosin-β-4(Tβ4) on an ischemic AKI model in rats. Methods Rats were randomly assigned into four groups (n = 8/group): The control group (sham-operated), the ischemia-reperfusion (I/R) group; renal ischemia (90 min) by infrarenal abdominal aortic occlusion followed by reperfusion (3 h), the Tβ4 + I/R group; treated with Tβ4 before I/R, and the I/Tβ4/R group; treated with Tβ4 just before reperfusion. Besides renal function determination (creatinine (Cr) and blood urea nitrogen (BUN)); histological evaluation was also conducted. Renal tissue caspase-9, matrix metalloproteinase (MMP-9) activities, and hyaluronan levels were measured. Additionally, renal tissue oxidative stress (lipid hydroperoxide, malondialdehyde, superoxide dismutase, glutathione, pro-oxidant-antioxidant balance, ferric reducing antioxidant power, nitric oxide), inflammation (tumor necrosis factor-α, interleukin-1β, interleukin-6, nuclear factor-κβ) were evaluated. Results I/R increased the level of caspase-9, MMP-9 activity, and hyaluronan (p
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