Sulforaphane alleviates methamphetamine-induced oxidative damage and apoptosis via the Nrf2-mediated pathway in vitro and in vivo

Autor: Qi Xiong, Xiang Tian, Weiling Li, Lin Chen, Mei Zhou, Congyue Xu, Qin Ru
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Food and Agricultural Immunology, Vol 31, Iss 1, Pp 859-880 (2020)
Druh dokumentu: article
ISSN: 0954-0105
1465-3443
09540105
DOI: 10.1080/09540105.2020.1784099
Popis: This research was designed to investigate if antioxidant sulforaphane (SFN) alleviates methamphetamine (METH)-induced neurotoxicity by activation of the Nrf2-mediated pathway. Oxidative damage, apoptosis and mitochondrial membrane potential were evaluated in PC12 cells or the prefrontal cortex of SD rats post METH treatment with/without SFN. In addition, knockdown of Nrf2 expression in PC12 cells was used to further study the underlying mechanism. The results showed that METH exposure induced increased oxidative damage and apoptosis accompanied by decreased expressions of Nrf2, HO-1 and GCS in PC12 cells and the prefrontal cortex of SD rats. Meanwhile SFN effectively prevented METH-induced cell oxidative damage, apoptosis and mitochondrial membrane potential; however, the knockdown of Nrf2 expression partly reversed the protective effect of SFN in reducing METH-induced oxidative damage and apoptosis in PC12 cells. These results indicate that SFN might be a promising functional food-derived compound for preventing METH-induced neurotoxicity via the Nrf2-mediated pathway.
Databáze: Directory of Open Access Journals
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