Pericellular Oxygen Monitoring during Low-Level Light Therapy in Cell Culture Using a Microsensor System

Autor: Julia Marzioch, Friederike Kramer, Peter Dungel, Jochen Kieninger, Gerald A. Urban
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Proceedings, Vol 1, Iss 4, p 499 (2017)
Druh dokumentu: article
ISSN: 2504-3900
DOI: 10.3390/proceedings1040499
Popis: An electrochemical microsensor system to monitor the pericellular oxygen concentration of fibroblasts during low-level light therapy in vitro was developed. The system provides in-sight into the metabolism of the cells during and in consequence of illumination with visible red light. This approach is a unique method for real-time investigations of cellular respiration during light therapy. The presented sensor system features direct amperometric measurements by using chronoamperometric protocols for long-term stability. The oxygen measurements do not show a disturbance by light.
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